Several decades later, the Yggdmillennia family estranged themselves from the Mage's Association and declare their independence. Enraged, the Mage’s Association sent out assassins, but the Servants they summoned turned against them.

Servants must fight against Servants.

The Holy Grail War system has changed, and a war of seven Knights versus seven Knights, the likes of which has never been seen before, has broken out. Thus, the curtain is raised on the first and most likely last Great Holy Grail War.

Sieg

Voice Actor: Natsuki Hanae

Sieg is a homunculus with the appearance of a young boy. Formed using the Einzbern techniques, he was cast to be a supply of magic by the Yggdmillennia family.

Ruler

Voice Actor: Maaya Sakamoto

Ruler was summoned by the Holy Grail to be the administrator for this Holy Grail War. She is a special servant of the Ruler class.

Shirou Kotomine

Voice Actor: Kouki Uchiyama

Shirou was dispatched by the Church to be another administrator for this Holy Grail War. A Catholic priest, he is the master of Assassin of Red.

Assassin of Red

Voice Actor: Kei Shindou

The Assassin of Red is a servant of the Assassin class who was summoned by Shirou Kotomine. Seemingly adrift in a decadent ambiance, she is an unrivaled beauty.

Kairi Sisigou

Voice Actor: Keiji Nomura

Kairi is a freelance necromancer. Employed by the Mage’s Association, he participates in this Holy Grail War. He is the master of the Saber of Red.

Saber of Red

Voice Actor: Miyuki Sawashiro

The Saber of Red is a servant of the Saber class and was summoned by Kairi Sisigou. This knight is overflowing with confidence to the point that it is excessive arrogance.