The twin sisters, Towa and Setsuna were separated from each other when they were caught in a forest fire as children. Towa goes into the tunnel of Jidaiju and travels through time from the Sengoku period to modern times. She was brought as a daughter of the Higurashi family, related to Kagome, and grows up to become a middle school girl talented in martial arts. Meanwhile, her sister Setsuna, who was left behind in the Sengoku period, works for Kohaku, who is the head of youkai extermination, and makes a living by killing youkai. Moroha is the daughter of Kagome and Inuyasha. She also slays youkai as a bounty hunter and is known as "Moroha the youkai slayer".

It's been 10 years since Towa and Setsuna were separated, and the tunnel of the Jidaiju has opened once again. Towa reunites with Setsuna, who appeared in the present day, but for some reason, she has forgotten about Towa. With Moroha, who came along with Setsuna, joining them, the three "Hanyou no Yashahime" ramp around both the modern age and the Sengoku period.

Towa Higurashi

Voice Actor: Sara Matsumoto

A modern-day child living in the Reiwa period. A 14-year-old middle school student. 10 years ago, she traveled through time through the tunnel of the Jidaiju. Kagome's brother, Souta, helps her and was brought up as his daughter. She is a talented martial artist, and gets in fights with the delinquents which leads to her transferring schools repeatedly. Now, she goes to Saint Gabriel Academy, which is a girls' school. On meeting with Setsuna and Moroha, who also came to the modern age, she tries to return to the Sengoku period to get back Setsuna's sleep. Her boyish clothes are for fighting. She is actually the daughter of Sesshomaru who is living in the Sengoku period.

TVアニメ『半妖の夜叉姫』の
”登場人物”をご紹介致します！

殺生丸の娘”日暮とわ”#半妖の夜叉姫 pic.twitter.com/uUK3mQuXbD

— 半妖の夜叉姫 (@hanyo_yashahime) May 9, 2020

Setsuna

Voice Actor: Mikako Komatsu

A 14-year-old girl. She makes a living by killing youkai under Kohaku, who is the head of the youkai extermination. She was separated from her twin sister, Towa, at the age of 4. 10 years later, she travels to the modern age along with Moroha, and reunites with Towa. However, she has no memory of her childhood since her sleep was stolen by the butterfly of dream. She doesn't believe that Towa is her older sister. Along with Towa and Moroha, who try to get back Setsuna's sleep, she travels back to the Sengoku period, but... Her calm and collected nature probably comes from her father, Sesshomaru.

TVアニメ『半妖の夜叉姫』の
”登場人物”をご紹介致します！

殺生丸の娘”せつな”#半妖の夜叉姫 pic.twitter.com/8TA5k1EIQ3

— 半妖の夜叉姫 (@hanyo_yashahime) May 9, 2020

Moroha

Voice Actor: Azusa Tadokoro

A 14-year-old bounty hunter. She sells the youkai she has slain (their heads and body parts) to Shikabaneya Juubee, who deals with youkai with bounties. Her sword is the cursed sword Kurikaramaru. She goes by the name of "Moroha the Youkai Slayer". By using the lipstick in the clamshell with a red pearl on it, she turns into "Kunikuzushi no Beniyasha" and goes berserk. She is cheerful and openhearted, and knows much of the world compared to Towa and Setsuna. She is actually the daughter of Inuyasha and Kagome, but doesn't know much about them because she lived on her own since she was a child.

TVアニメ『半妖の夜叉姫』の
”登場人物”をご紹介致します！

殺生丸の娘”せつな”#半妖の夜叉姫 pic.twitter.com/8TA5k1EIQ3

— 半妖の夜叉姫 (@hanyo_yashahime) May 9, 2020